WebFeb 18, 2024 · Highly efficient gene editing was also achieved in watermelon (Citrullus lanatus) with the combination of GRF4-GIF1 and CRISPR/Cas9 gene editing tool (Feng et al. 2024). In this study, they … WebOct 12, 2024 · The following day we added 300 μl of the Agrobacterium culture to 20 ml TY (pH 5.5) containing the appropriate antibiotics and 200 μM acetosyringone. We incubated the culture 28 °C for in a shaking incubator set at 250 rpm until the culture reached an OD 600 between 0.1 – 0.2 (approximately 2–4 h).

Highly efficient, genotype-independent transformation …

WebThese advances have enabled an in-depth understanding of their domestication and evolution, and facilitated the genetic dissection of numerous agronomic traits, which jointly expedites the exploitation of state-of-the-art biotechnologies in vegetable breeding. WebTaGRF4-GIF1: ZmUbi: Triticum aestivum immature embryo: Increased regeneration efficiency 7.8 times; shortened protocol : O. sativa calli from seeds: Increased … lake tahoe teams background

‪Li Yuan‬ - ‪Google Scholar‬

WebNote the growing green shoots in the callus transformed with the wheat GRF4–GIF1 chimera in the absence of cytokinins (red arrows). ... Overexpressing a chimeric fusion of ClGRF4 and ClGIF1 ... WebHighly efficient, genotype‐independent transformation and gene editing in watermelon ( Citrullus lanatus ) using a chimeric ClGRF4‐GIF1 gene WebDec 18, 2024 · Similarly, the GRF4-GIF1 chimeras from Citrus and grape (Vitis vinifera), respectively, enhanced the regeneration ability of dicotyledonous citrus. Mutations in the miR396 target site further elevated the activity of the grape GRF4-GIF1 chimera. The superior performance in both monocots and dicots highlights the broad application … lake tahoe swimming pictures