Polysome profiling and cancer

Author: feem

August undefined, 2024

WebSep 18, 2024 · Polysome profiling analysis of the early passage Eμ-Myc lymphoma “CX” cell lines and investigating potential roles of RAPGEF3 (EPAC1) and RAPGEF4 (EPAC2) in human blood cancers A. Enrichment analysis by GeneGO MetaCore ® of the polysomal RNA-seq data comparing CX-5461-resistant (CX) and drug-naïve cells (CTRL; n = 3; false discovery … WebMetformin caused a dose-dependent specific decrease in cap-dependent translation, with a maximal inhibition of 40%. Polysome profile analysis showed an inhibition of translation initiation as metformin treatment of MCF-7 cells led to a shift of mRNAs from heavy to light polysomes and a concomitant increase in the amount of 80S ribosomes.

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WebMar 7, 2024 · Polysome profiling analysis shows that MYC oncoprotein synthesis strictly depends on CDK13-regulated translation in colorectal cancer (CRC), and CDK13 is … WebOct 26, 2024 · Polysome fractionation workflow: After cycloheximide pre-treatment, cells are lysed (a) and cytoplasmic extracts are loaded onto sucrose gradients where polysomes, … inception maxpooling

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WebI lead a multi-disciplinary research team composed of research professionals and trainees (i.e. students, postdocs) at different career stages with broad expertise in molecular biology, biochemistry, genomics and bio-informatics. We are primarily focused on studying the biological functions and mechanisms of intracellular RNA transport, as well as their … WebJan 1, 2024 · Polysome profiling involves separation of cellular components by density gradient centrifugation allowing for separation of 40S and 60S ribosomal subunits from ... This work was supported by a Canadian Cancer Society Research Institute Innovation Grant and a CIHR Project Grant. D. G. was supported by the NSERC USRA program ... WebMay 12, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating the response of glioblastoma to ionizing radiation (IR), we used polysome profiling to define the IR-induced translatomes of a set of human glioblastoma stem-like cell (GSC) lines. inception maze

Carolyn Jones - Research Assistant - University of Leicester

mTOR-sensitive translation: Cleared fog reveals more trees

WebDec 9, 2015 · Dynamic & enthusiastic Medical Researcher highly regarded for utilizing strong analytical skills and extensive laboratory experience to further advance within neuroscience and conduct cutting-edge cancer biology research. Strong background in molecular and cell biology, advanced microscopy, human/mice genetics, and bioinformatics. Broad … WebApr 11, 2024 · The disease is the leading cause of cancer death, according to the American Cancer Society, which estimates that this year there will be more than 238,000 new cases of lung cancer and more than ... inception main themeWebOct 7, 2016 · The polysome profile of fertilized embryos and the corresponding RNA profile of the 15–40% sucrose gradient separated on an agarose gel are shown in Figure 3B. In the first fractions, only low molecular weight RNAs were visible, then fractions containing only 40S subunits were isolated as shown by the presence of the 18S rRNA band, followed by … inception matt damon

"WebMay 17, 2014 · An example of applying the polysome fractionation method to investigate the role of mTOR signaling in mediating the effects of insulin on mRNA translation is presented. To this end, MCF7 human breast cancer cells were maintained in low serum and then stimulated with insulin alone or in combination with the active-site mTOR inhibitor Torin1. " - Polysome profiling and cancer

Polysome profiling and cancer

IJMS Free Full-Text Polysome Profiling of a Human …

WebSep 16, 2024 · Polysome profiling of cells treated with three hypothetical translation inhibitors is shown in the plots below. These three inhibitors are: (i) ... The cancer cells differentiated into a mesenchymal phenotype and grew in fresh culture medium containing inhibitors of epithelial-to-mesenchymal transition (EMT). WebLearned many techniques such as Cloning, Western blotting, Luciferase Assay, Polysome profiling, Plasmid and Genomic DNA Isolation, PAGE, Polymerase Chain Reaction, Site-directed ... Worked in Gamma Radiobiology lab on the project titled,"Bone marrow cancer treatment through Emergency drug testing(IP,IV) in mice by bone marrow ...

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WebMay 12, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating … WebJan 9, 2024 · Polysome-profiling is commonly used to study translatomes and applies laborious extraction of efficiently translated mRNA (associated with >3 ribosomes) from a …

WebMay 15, 2016 · Changes in polysome-bound mRNA (translatome) are correlated closely with changes in the proteome in cells. Therefore, to better understand the processes mediating … WebRibosome profiling is a method based on deep sequencing of ribosome-protected mRNA fragments. Ribosome profiling, also known as Ribo-Seq (ribosome sequencing) or ART-Seq (active mRNA translation sequencing), provides a “snapshot” of all the ribosomes active in a cell at a specific time point. This information can help researchers determine ...

WebIntroduction. Studies into the genomics, epigenetics, and transcriptomes of cancer have yielded important insights into its pathogenesis. However, proteomic studies revealed that the transcriptome does not always predict the proteome (de Sousa Abreu et al, 2009).This disconnect is due, in part, to post-transcriptional regulation, e.g., splicing, nuclear RNA … WebOct 29, 2024 · Beatrice Hunter Cancer Research Institute, Halifax, Nova Scotia, Canada. Corresponding author: [email protected] Search for more papers by this author. ...

WebMiR-210 is induced by hypoxia and plays different roles in the development of certain cancers. However, little is known about its role in pancreatic cancer (PC). This study aimed to explore the induction and modulation of PC by miR-210 and its potential molecular targets. Methods.

WebPolysome-profiling revealed that one third of protein-coding mRNAs expressed in primary mouse macrophages are differentially translated upon infection with L. donovani promastigotes or ... Rapamycin can stimulate HSV1 replication in cancer cells, but active-site dual mTORC1 and mTORC2 (mammalian target of rapamycin complex 1 and 2 ... inception mccoy tynerWebMy specialist areas are Ion Torrent PGM sequencing, using Ampliseq and RNA-seq in particular. This work goes towards cancer mutation analysis and target identification. Other skills include polysome profiling, for gene expression and miRNA studies. My key responsibilities are to manage the… Show more inabel characteristicsWebFeb 19, 2024 · Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive. Repetitive polysome fractionation rapidly generates a large number of samples to be handled in the downstream processes of protein elimination, RNA extraction and quantification. Here, we … inabia solutions and consultingWebI am processing samples for polysome profiling, and I've been getting great profiles for the primary cancer cell lines I've been working, but I have had trouble with the mouse embryonic fibroblast ... inception meanWebExploring the field of epitranscriptomics and its implications in human cancer cell lines to identify potential drug targets. • Generated NSUN2 KO human cancer cell lines using CRISPR- Cas9. • Performed downstream analysis of NSUN2 KO cells (polysome and ribosome profiling and sequencing) and I am currently working on the functional validation. inception mal cobbWebApr 8, 2024 · Polysome profiling is a powerful method to study protein translation regulation. It allows to measure the translational status of individual mRNAs or examine … inabia software \u0026 consulting incWeb2 Beatrice Hunter Cancer Research Institute, Halifax, Nova Scotia, Canada. PMID: 30371019 ... Polysome profiling analysis utilizes ultracentrifugation to isolate complexes of mRNAs … inception meaning in chinese